| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.076929 |
| Chromosome: | chromosome 2 |
| Location: | 1754294 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g086500 | PGM10 | (1 of 2) PTHR23029//PTHR23029:SF24 - PHOSPHOGLYCERATE MUTASE // SUBFAMILY NOT NAMED; Phosphoglycerate mutase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGAGCATCTGCATGTCAAGACTGTCAGAGC |
| Internal bar code: | AGAACTTGATGCTAGGATCATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 215 |
| LEAP-Seq percent confirming: | 97.1499 |
| LEAP-Seq n confirming: | 784 |
| LEAP-Seq n nonconfirming: | 23 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTGTGCTGTTGTGTGTGAG |
| Suggested primer 2: | GCTGGAGCCCTACTGTCAAC |