| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.076946 |
| Chromosome: | chromosome 3 |
| Location: | 2172220 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g157400 | (1 of 1) IPR000595//IPR014710//IPR018490 - Cyclic nucleotide-binding domain // RmlC-like jelly roll fold // Cyclic nucleotide-binding-like | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCATCGGGCTCGGGTATGTAACCCCACGC |
| Internal bar code: | TATGCTTGAAGACCCCTTCGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1208 |
| LEAP-Seq percent confirming: | 99.7387 |
| LEAP-Seq n confirming: | 1145 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCTTGTGACGCGTTTGTGT |
| Suggested primer 2: | TAGGAGGTTGTGCGAGTGGT |