| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.076954 |
| Chromosome: | chromosome 5 |
| Location: | 3406354 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g241150 | (1 of 1) K11491 - condensin-2 complex subunit D3 (NCAPD3) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCTGGATGGTGGGTGTGCCGCTCGTGTGT |
| Internal bar code: | CAGGATCCCGTAGCTCGGCACA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 248 |
| LEAP-Seq percent confirming: | 99.2443 |
| LEAP-Seq n confirming: | 1970 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAAACCAAAACCAACGAGGG |
| Suggested primer 2: | CACCTTCATCGCAGCAGAC |