Insertion junction: LMJ.RY0402.076954_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.076954
Chromosome:chromosome 9
Location:2470394
Confidence (%):73
Locus systematic id Locus common name Defline Feature
intergenic

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATCACACACCGACCGCCCCCCCCCCCCAAA
Internal bar code:TTTTAGAATGGGTTTACAAGAA

Confirmation - LEAP-Seq

LEAP-Seq distance:155
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:34
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:CACCACAACCATTCAAGTGC
Suggested primer 2:ACAAAATGCTAGACCCCGTG