| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.077016 |
| Chromosome: | chromosome 10 |
| Location: | 2121484 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g433400 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGCACGGTCATGGCTTGAAGGCGAAACCC |
| Internal bar code: | GATCGACGTGAGACGCAAGTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 228 |
| LEAP-Seq percent confirming: | 97.8928 |
| LEAP-Seq n confirming: | 2648 |
| LEAP-Seq n nonconfirming: | 57 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACACACACACACACAAGCC |
| Suggested primer 2: | GATCAAGTCACCAAGGGCAT |