| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.077035 |
| Chromosome: | chromosome 12 |
| Location: | 1716201 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g512750 | TEF20 | Notchless-like protein; (1 of 1) PTHR22847//PTHR22847:SF50 - WD40 REPEAT PROTEIN // SUBFAMILY NOT NAMED | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCATATCCAGACCCTAGCAACCATTACAC |
| Internal bar code: | CTGTCTCTGGGAACGCGGCACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 337 |
| LEAP-Seq percent confirming: | 82.082 |
| LEAP-Seq n confirming: | 6505 |
| LEAP-Seq n nonconfirming: | 1420 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGGCACAGCAAGATTCACA |
| Suggested primer 2: | AGTGGCTAAGTGCAGCCTGT |