| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.077036 |
| Chromosome: | chromosome 3 |
| Location: | 2059903 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g156550 | (1 of 1) PTHR22829//PTHR34826 - DEP DOMAIN PROTEIN // FAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGTACACAATGTGCGTTCCAAGCCTCACC |
| Internal bar code: | GCTCGCAGCAAGATGCGTGTGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 505 |
| LEAP-Seq percent confirming: | 95.251 |
| LEAP-Seq n confirming: | 2467 |
| LEAP-Seq n nonconfirming: | 123 |
| LEAP-Seq n unique pos: | 30 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGACAGCGACTGTAGAAGC |
| Suggested primer 2: | AAGAAGTGAAGGCGTAGCCA |