Insertion junction: LMJ.RY0402.077043_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.077043
Chromosome:chromosome 13
Location:2539670
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre13.g580600 ATT1,ATE1 Arginyl-tRNA protein transferase; (1 of 1) 2.3.2.8 - Arginyltransferase / Arginyl-tRNA--protein transferase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGGCGCGGAAGCTGCTGACGAGCTGCGTCG
Internal bar code:TGGGCTAAAAATAATGCGGCAA

Confirmation - LEAP-Seq

LEAP-Seq distance:568
LEAP-Seq percent confirming:99.1476
LEAP-Seq n confirming:2210
LEAP-Seq n nonconfirming:19
LEAP-Seq n unique pos:14

Suggested primers for confirmation by PCR

Suggested primer 1:CTTTGCCTTGACCTGTCCTC
Suggested primer 2:GTACCAGGTGGAGGTGCTGT