Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.077061 |
Chromosome: | chromosome_7 |
Location: | 2877062 |
Confidence (%): | 58 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre07.g332300 | 1,GWD2 | R1 Protein, alpha-glucan water dikinase | sense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | ATATACTGAGCCATCCCGACCCTTCCCGAC |
Internal bar code: | TAGCCGGCCGGGTATCTAGCGC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 77 |
LEAP-Seq percent confirming: | 82.3623 |
LEAP-Seq n confirming: | 2064 |
LEAP-Seq n nonconfirming: | 442 |
LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GTTGGGAGTAGGTCGTGAGC |
Suggested primer 2: | TTTGGTTTAGTTTGGGCTGG |