| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.077061 |
| Chromosome: | chromosome 7 |
| Location: | 2877062 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g332300 | GWD2,1 | Alpha-glucan water dikinase 2; (1 of 3) 2.7.9.4 - Alpha-glucan, water dikinase / Starch-related R1 protein | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATATACTGAGCCATCCCGACCCTTCCCGAC |
| Internal bar code: | TAGCCGGCCGGGTATCTAGCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 77 |
| LEAP-Seq percent confirming: | 82.3623 |
| LEAP-Seq n confirming: | 2064 |
| LEAP-Seq n nonconfirming: | 442 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTGGGAGTAGGTCGTGAGC |
| Suggested primer 2: | TTTGGTTTAGTTTGGGCTGG |