| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.077065 |
| Chromosome: | chromosome 7 |
| Location: | 3312132 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g334600 | CGL20 | (1 of 1) PTHR35713//PTHR35713:SF1 - FAMILY NOT NAMED // GENOMIC DNA, CHROMOSOME 3, P1 CLONE: MOB24; Conserved in the Green Lineage | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGCATGGGAGCCTCCCATTCCTGCGGCGC |
| Internal bar code: | CAGTATGCCCGAATCGGGGTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 218 |
| LEAP-Seq percent confirming: | 93.8128 |
| LEAP-Seq n confirming: | 1213 |
| LEAP-Seq n nonconfirming: | 80 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCTGCAAACCTTCATTCTG |
| Suggested primer 2: | CTCCTCCTTCCTTGCTGATG |