| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.077068 |
| Chromosome: | chromosome 9 |
| Location: | 3985571 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g392729 | (1 of 2) 2.1.2.9 - Methionyl-tRNA formyltransferase / transformylase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGGGCAGGGAGCGGGAGGAAGGGCGCGAG |
| Internal bar code: | CTGTATGTGGATTCCTTACTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 505 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 263 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCCGCTAATATCCTCGCTG |
| Suggested primer 2: | AGAAATACAGGGGCAACACG |