Insertion junction: LMJ.RY0402.077069_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.077069
Chromosome:chromosome 8
Location:1658106
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre08.g365632 PPP32 Phosphoprotein phosphatase 2C-related; (1 of 70) 3.1.3.16 - Protein-serine/threonine phosphatase / Serine/threonine specific protein phosphatase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCTGCCGTACCACTATGCCTAGCTACAACT
Internal bar code:TGTCGAGCCGGAAGGTGCGTTG

Confirmation - LEAP-Seq

LEAP-Seq distance:74
LEAP-Seq percent confirming:99.3666
LEAP-Seq n confirming:1255
LEAP-Seq n nonconfirming:8
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:TCCTCCTCACCCTCTCTCAA
Suggested primer 2:ATCCTGTCAGGCACTCTGCT