| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.077089 |
| Chromosome: | chromosome 1 |
| Location: | 5015087 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g034600 | (1 of 1) PTHR22847:SF430 - MZB10.11 PROTEIN-RELATED | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGGCGAAGGAGGGCCCCGACGCTTGGGTA |
| Internal bar code: | GGGCGAGAGAGGGCGTAGAGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 556 |
| LEAP-Seq percent confirming: | 98.7526 |
| LEAP-Seq n confirming: | 950 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTGCGGGTGTATTGGTAGC |
| Suggested primer 2: | TACTCGCACAGCCAACTCAC |