| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.077105 |
| Chromosome: | chromosome 6 |
| Location: | 6971312 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g296400 | (1 of 1) 1.3.8.4 - Isovaleryl-CoA dehydrogenase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCAAATTGCGCGGAAAGCGTTTCGGAGAAG |
| Internal bar code: | TACTGTTACCTAAAGTCGGAAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 746 |
| LEAP-Seq percent confirming: | 99.7206 |
| LEAP-Seq n confirming: | 7138 |
| LEAP-Seq n nonconfirming: | 20 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCACGACTTCTCAACGCAA |
| Suggested primer 2: | GTGTAGACGTTTTTGGCCGT |