| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.077111 |
| Chromosome: | chromosome 4 |
| Location: | 1537581 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g213652 | TERT1,TERT,CrTERT | Telomerase reverse transcriptase; (1 of 1) K11126 - telomerase reverse transcriptase (TERT, EST2) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCCTGTTTGGCTGCGGGTGCATACGGTTC |
| Internal bar code: | CCAGCCCCGTGTGAGTTCTCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 460 |
| LEAP-Seq percent confirming: | 94.7282 |
| LEAP-Seq n confirming: | 44096 |
| LEAP-Seq n nonconfirming: | 2454 |
| LEAP-Seq n unique pos: | 132 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGAGTGGGGCACAAAGTAGA |
| Suggested primer 2: | AACACATTTGCACACGCAAT |