Insertion junction: LMJ.RY0402.077114_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.077114
Chromosome:chromosome 11
Location:1013724
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre11.g467666 LCI13,GT90F43,GT90-43 Low-CO2-inducible 13; (1 of 52) PF05686 - Glycosyl transferase family 90 (Glyco_transf_90) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGGGGACAGCCATGCGCAACATTTGCCAAC
Internal bar code:TTTTACTTGAGTATGCGGTTTT

Confirmation - LEAP-Seq

LEAP-Seq distance:325
LEAP-Seq percent confirming:67.8626
LEAP-Seq n confirming:2232
LEAP-Seq n nonconfirming:1057
LEAP-Seq n unique pos:14

Suggested primers for confirmation by PCR

Suggested primer 1:AGAAGCTCCCAGTACCAGCA
Suggested primer 2:GGGCTGGCTTGACATTAAAA