| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.077130 |
| Chromosome: | chromosome 17 |
| Location: | 765212 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g701500 | DNJ1 | (1 of 1) K09503 - DnaJ homolog subfamily A member 2 (DNAJA2); DnaJ-like protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTAAATCGAGCTTCTGTACTTTTGTTACG |
| Internal bar code: | ATTGGCCAGGACCTTGTCCGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 157 |
| LEAP-Seq percent confirming: | 99.7616 |
| LEAP-Seq n confirming: | 837 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTATCTGCCATCATCCCCTG |
| Suggested primer 2: | TAGTCAAGGGGTCAACAGGG |