| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.077138 |
| Chromosome: | chromosome 12 |
| Location: | 4893694 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g525350 | PTK15 | (1 of 274) IPR020683 - Ankyrin repeat-containing domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGTGACCAGTGTACAGCGGCCACGAGTCCT |
| Internal bar code: | ACCAATGTCCGCTAAGAATGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1120 |
| LEAP-Seq percent confirming: | 99.6516 |
| LEAP-Seq n confirming: | 16589 |
| LEAP-Seq n nonconfirming: | 58 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATTTTGACGGTCTAGGGGCT |
| Suggested primer 2: | AGGTCGCTATGGGTGAACAG |