| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.077143 |
| Chromosome: | chromosome 10 |
| Location: | 1156881 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g425800 | ORN1 | (1 of 1) 3.1.13.3 - Oligonucleotidase; Putative organellar oligoribonuclease | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGTCCACCCATACAAGCGGGTTCCGCAGT |
| Internal bar code: | GGGGTACGTTGGGATATTGGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1184 |
| LEAP-Seq percent confirming: | 99.8363 |
| LEAP-Seq n confirming: | 7318 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACTGCGGCTTAGTCCGTCT |
| Suggested primer 2: | GCTGTTGTCTCCTCCCTCTG |