| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.077151 |
| Chromosome: | chromosome 16 |
| Location: | 5429450 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g681200 | CDS/3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTAGACACGGAAGATGAGCTTGACACGGTA |
| Internal bar code: | TTAGGTGGGTGGTTCGGATGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 613 |
| LEAP-Seq percent confirming: | 99.4516 |
| LEAP-Seq n confirming: | 1088 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCTTTGGGCAGCCTGTAAG |
| Suggested primer 2: | ATGCAACATTTACGCCAACA |