| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.077163 |
| Chromosome: | chromosome_6 |
| Location: | 1167666 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre06.g257250 | BBS2 | Bardet-Biedl syndrome-2 associated protein | antisense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TGGCCGACACGTTGTCGCCCGTGACCGTCC |
| Internal bar code: | ACGGTCGGGCGAAAGGTAGTAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 176 |
| LEAP-Seq percent confirming: | 65.586 |
| LEAP-Seq n confirming: | 263 |
| LEAP-Seq n nonconfirming: | 138 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TATGCGCACTCTGACTCACC |
| Suggested primer 2: | GTGCTACGATGTGGAGCTGA |