| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.077165 |
| Chromosome: | chromosome 10 |
| Location: | 1085012 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g425350 | (1 of 6) IPR001245//IPR011009 - Serine-threonine/tyrosine-protein kinase catalytic domain // Protein kinase-like domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACATCTAGACCAATACCTGTGGCGGGCTGC |
| Internal bar code: | AACCAGATCGTTCATGCTCGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 686 |
| LEAP-Seq percent confirming: | 99.4152 |
| LEAP-Seq n confirming: | 4250 |
| LEAP-Seq n nonconfirming: | 25 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGTGTGATATGTGTCCGAG |
| Suggested primer 2: | AGGGTTTAGGCAGGCGTATT |