Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.RY0402.077196 |
Chromosome: | chromosome_9 |
Location: | 7189068 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre09.g412175 | sense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | ATGATGATGACGCCCTCACCTCGTGACGCA |
Internal bar code: | CCGTAGAGGGCTTGATATTGTT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 270 |
LEAP-Seq percent confirming: | 99.6678 |
LEAP-Seq n confirming: | 600 |
LEAP-Seq n nonconfirming: | 2 |
LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GTTTGGATCCAGCCAGTGAT |
Suggested primer 2: | TGGTCGGTAAGAGGGATGAG |