Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.RY0402.077200 |
Chromosome: | chromosome_16 |
Location: | 494114 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre16.g693204 | antisense | 3'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GTCGGCTGTAGGGCCGTGTTGCGGAAATGA |
Internal bar code: | GGTAAACTCGCTGGGTACGCGG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 449 |
LEAP-Seq percent confirming: | 98.2213 |
LEAP-Seq n confirming: | 497 |
LEAP-Seq n nonconfirming: | 9 |
LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CGCTGTTCAACGAGATTTCA |
Suggested primer 2: | CGGAACTGTTGGTTCTGGTT |