| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.077241 |
| Chromosome: | chromosome 2 |
| Location: | 7440113 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g144450 | FAP1 | Flagellar Associated Protein 1 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGAGGATGCTGACAGCTGTCAGCTTAGGGG |
| Internal bar code: | GCCACCACGTGGCATGGCTAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 695 |
| LEAP-Seq percent confirming: | 99.8468 |
| LEAP-Seq n confirming: | 2607 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTTCTTAGAGCGCCTGTGG |
| Suggested primer 2: | GCTGCAAGGGAAGAGAAATG |