Insertion junction: LMJ.RY0402.077242_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.077242
Chromosome:chromosome 4
Location:2128259
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre04.g219600 (1 of 9) IPR000210//IPR011042//IPR011333 - BTB/POZ domain // Six-bladed beta-propeller, TolB-like // POZ domain 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCAGAAGTGCCAGCCATTGTAACGACTGAT
Internal bar code:AGTAAACTCCGGGGGCATACCG

Confirmation - LEAP-Seq

LEAP-Seq distance:846
LEAP-Seq percent confirming:99.6764
LEAP-Seq n confirming:5545
LEAP-Seq n nonconfirming:18
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:ACCGTCACCTTCCATACTGC
Suggested primer 2:AAGGATACCCGTGTCTGTGC