| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.077254 |
| Chromosome: | chromosome 17 |
| Location: | 3558943 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g725450 | (1 of 1) K11789 - HIV-1 Vpr-binding protein (VPRBP, DCAF1) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAACGAGCGCGTGGTGAGGTCGGTGTGTAA |
| Internal bar code: | GCATCGTGATACCGTGCCGAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 394 |
| LEAP-Seq percent confirming: | 98.2669 |
| LEAP-Seq n confirming: | 5103 |
| LEAP-Seq n nonconfirming: | 90 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGGCTCGTCTGTCTTTTCC |
| Suggested primer 2: | GCGACACACAAAAACACCAC |