| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.077262 |
| Chromosome: | chromosome 3 |
| Location: | 1653626 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g153050 | RWP1 | (1 of 1) IPR000104//IPR003035 - Antifreeze protein, type I // RWP-RK domain; RWP-RK transcription factor | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCAAGACGACGTCGGTTCGCCTGCGGAAGC |
| Internal bar code: | CCCAACGTCAGGTAGTCGCTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 468 |
| LEAP-Seq percent confirming: | 99.213 |
| LEAP-Seq n confirming: | 4160 |
| LEAP-Seq n nonconfirming: | 33 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AATTTTTGACGGTGAGGTCG |
| Suggested primer 2: | GCTGTTGAACCAAAAGCACA |