| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.077272 |
| Chromosome: | chromosome 13 |
| Location: | 548680 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g565301 | intron|outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGGAACACACCAAGTCAGTGAGCCAGCAG |
| Internal bar code: | GTCACGTCCTGGCTGGGCTTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 927 |
| LEAP-Seq percent confirming: | 99.4783 |
| LEAP-Seq n confirming: | 7627 |
| LEAP-Seq n nonconfirming: | 40 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACTTACTTGCCCCCACCTA |
| Suggested primer 2: | GCAAACTACAGGCAACAGCA |