| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.077280 |
| Chromosome: | chromosome 7 |
| Location: | 3644381 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g337100 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGCGCGTAAGCCCTCAGCTTTCTTGTCTT |
| Internal bar code: | GTGGATCCAGGGGGTGCGGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 639 |
| LEAP-Seq percent confirming: | 99.3927 |
| LEAP-Seq n confirming: | 3928 |
| LEAP-Seq n nonconfirming: | 24 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAACAAGCTTCGCAAAGGAC |
| Suggested primer 2: | GTCCGGTGAGTTCCTGATGT |