| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.077280 |
| Chromosome: | chromosome 7 |
| Location: | 3644394 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g337100 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGGTAGAGGAGCTAGGATGGGAGTCAGAG |
| Internal bar code: | CACCAGTGGGCGCCAGGCTCTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 656 |
| LEAP-Seq percent confirming: | 97.822 |
| LEAP-Seq n confirming: | 1572 |
| LEAP-Seq n nonconfirming: | 35 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCCGGTGAGTTCCTGATGT |
| Suggested primer 2: | GAACAAGCTTCGCAAAGGAC |