| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.077298 |
| Chromosome: | chromosome 12 |
| Location: | 1578076 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g486650 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCTAGGCCATGTGGCACACATGCAAGGGG |
| Internal bar code: | CGGAAAGGCTCGATATCGCTAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 727 |
| LEAP-Seq percent confirming: | 97.5041 |
| LEAP-Seq n confirming: | 2969 |
| LEAP-Seq n nonconfirming: | 76 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACCAGGATGTGCTGGATCT |
| Suggested primer 2: | AACAGATGACGGGAATACGG |