| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.077321 |
| Chromosome: | chromosome 12 |
| Location: | 6069403 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g535476 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCCTCGTCTCAACTGCTTCCCTGACTCTG |
| Internal bar code: | GGATTCATGGGAATATCTAATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 561 |
| LEAP-Seq percent confirming: | 99.2828 |
| LEAP-Seq n confirming: | 2215 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACCGATAGAACGCAGGATG |
| Suggested primer 2: | CAGGATGTGAAGTGGGACCT |