| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.077354 |
| Chromosome: | chromosome 16 |
| Location: | 2448222 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g660601 | (1 of 1) IPR000772//IPR003609 - Ricin B, lectin domain // PAN/Apple domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCTTCTACCCCTGGCGCAAGGATTTATCC |
| Internal bar code: | GCCCCGCGAAGCCCCTTAAAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 213 |
| LEAP-Seq percent confirming: | 98.8837 |
| LEAP-Seq n confirming: | 5935 |
| LEAP-Seq n nonconfirming: | 67 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCAGCAGTCATCCAGAAAC |
| Suggested primer 2: | TCACTTGAAAGCACGGTCTG |