Insertion junction: LMJ.RY0402.077359_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.077359
Chromosome:chromosome 2
Location:2420114
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre02.g091500 (1 of 1) PTHR13595:SF1 - ADP-RIBOSYLATION FACTOR-LIKE PROTEIN 6-INTERACTING PROTEIN 4 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTCTCGATCGCCGAGGCGCACTTCTGAAAC
Internal bar code:CCAAGCATGTCCACGGGTAATT

Confirmation - LEAP-Seq

LEAP-Seq distance:507
LEAP-Seq percent confirming:98.6207
LEAP-Seq n confirming:143
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:CGTTTGCAAATCGTAAGGGT
Suggested primer 2:TCGGCAGGAGTTTCTCATTT