| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.077372 |
| Chromosome: | chromosome 2 |
| Location: | 531001 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g077150 | CPLD14 | Conserved in the Plant Lineage and Diatoms; (1 of 2) PF05684 - Protein of unknown function (DUF819) (DUF819) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCAAGGTTAAAACCGGTGGGAACAGGATA |
| Internal bar code: | GAGCTCGTGGCTCGTGCGCCGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 530 |
| LEAP-Seq percent confirming: | 99.8828 |
| LEAP-Seq n confirming: | 3409 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGTAACGACCCAACCGTCT |
| Suggested primer 2: | GCAGGGTTGTCAGGTTGAAT |