| Insertion cassette: | CIB1 | 
| Side of cassette: | 3' | 
| Strand: | - | 
| Strain: | LMJ.RY0402.077414 | 
| Chromosome: | chromosome 14 | 
| Location: | 1711195 | 
| Confidence (%): | 73 | 
| Locus systematic id | Locus common name | Defline | Feature | 
|---|---|---|---|
| Cre14.g619450 | CDS | 
| Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGGCTCGTCGCCGGGGAAGAGCGGGGGAG | 
| Internal bar code: | ACGCAGGATCGCACGCGCCAGG | 
| Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 535 | 
| LEAP-Seq percent confirming: | 98.9123 | 
| LEAP-Seq n confirming: | 2910 | 
| LEAP-Seq n nonconfirming: | 32 | 
| LEAP-Seq n unique pos: | 15 | 
| Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATGTGGTCAGCAACGTACC | 
| Suggested primer 2: | ATTACAACAAGCCACCCAGC |