Insertion junction: LMJ.RY0402.077432_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.077432
Chromosome:chromosome 6
Location:2762416
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre06.g270900 SUV3 Mitochondrial DEAD-box DNA/RNA helicase; (1 of 1) K17675 - ATP-dependent RNA helicase SUPV3L1/SUV3 [EC:3.6.4.13] (SUPV3L1, SUV3) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACGCCTCCCGCCCCGTGCGGCGCACCACCT
Internal bar code:GTGCGGATGCACCACTCTCGCC

Confirmation - LEAP-Seq

LEAP-Seq distance:821
LEAP-Seq percent confirming:99.1826
LEAP-Seq n confirming:364
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:GAGGTCTGCGTGACTGGAAT
Suggested primer 2:TGAGTCCTGGAAACCACACA