| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.077432 |
| Chromosome: | chromosome 10 |
| Location: | 398244 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g420561 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGTACGTGCTGTGTGACGGCGCGGCCCGC |
| Internal bar code: | GGAGCAGGCGTGAGGCGGTGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 394 |
| LEAP-Seq percent confirming: | 99.3161 |
| LEAP-Seq n confirming: | 2033 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTTGACCGCCAGTGAAGTT |
| Suggested primer 2: | GGGTCCACAAAGAGGTCGTA |