| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.077445 |
| Chromosome: | chromosome 9 |
| Location: | 913182 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g401150 | NUOFAF2 | (1 of 1) K18160 - NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 2 (NDUFAF2); NADH:ubiquinone oxidoreductase 1 alpha subcomplex assembly factor 2 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTTTCAGTGGGAGACGGCAGCTGCGCGAG |
| Internal bar code: | GGTTGTTCATCTTCTCCCGATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 717 |
| LEAP-Seq percent confirming: | 99.322 |
| LEAP-Seq n confirming: | 3809 |
| LEAP-Seq n nonconfirming: | 26 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAAGTGTCCGGAATTTCGTT |
| Suggested primer 2: | GTCTAATGCACCCGTGACCT |