| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.077449 |
| Chromosome: | chromosome 6 |
| Location: | 6617524 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g293750 | (1 of 19) PF00642 - Zinc finger C-x8-C-x5-C-x3-H type (and similar) (zf-CCCH) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AACCAACCGTATCTGTGGTGTATGTATGTA |
| Internal bar code: | GATTGTACCCGTGCTGCGTCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 466 |
| LEAP-Seq percent confirming: | 89.313 |
| LEAP-Seq n confirming: | 468 |
| LEAP-Seq n nonconfirming: | 56 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAGCCTTGCGATAGCTCCAT |
| Suggested primer 2: | GCTGTACGCTGAGACGATCA |