| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.077460 |
| Chromosome: | chromosome 3 |
| Location: | 6627386 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g196750 | (1 of 4) PF01679 - Proteolipid membrane potential modulator (Pmp3) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTACCTCCTCGGTAGCTGCAACACGCGCAC |
| Internal bar code: | CGTTTGTTTGGCTGCTACGTGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 218 |
| LEAP-Seq percent confirming: | 99.584 |
| LEAP-Seq n confirming: | 1915 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCACAGTGCGACACGTTAC |
| Suggested primer 2: | TTGGCAGTGTGAGAATGAGC |