| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.077466 |
| Chromosome: | chromosome 11 |
| Location: | 1620101 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467766 | (1 of 1) 6.5.1.3 - RNA ligase (ATP) / Ribonucleic ligase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTGCGTGCACAGGTGTGCCGGGCGGGGGT |
| Internal bar code: | ACAAAACCCGTGTGGGCCAAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 597 |
| LEAP-Seq percent confirming: | 89.1089 |
| LEAP-Seq n confirming: | 90 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAGGCGTGTAGGTGGGTAGG |
| Suggested primer 2: | CCATGGGATGGTGTGTATCA |