Insertion junction: LMJ.RY0402.077473_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.077473
Chromosome:chromosome 1
Location:86547
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre01.g000600 (1 of 1) PTHR19848//PTHR19848:SF0 - WD40 REPEAT PROTEIN // NOTCHLESS PROTEIN HOMOLOG 1; Predicted WD-4 repeat family protein / notchless protein, putative intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTGTGTCACACCCGTGTGCCACATCCCACC
Internal bar code:CAGACCGGCAGAAGGCGCTCTA

Confirmation - LEAP-Seq

LEAP-Seq distance:304
LEAP-Seq percent confirming:99.0698
LEAP-Seq n confirming:1065
LEAP-Seq n nonconfirming:10
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:GTGCCAGTACATGCGAGAGA
Suggested primer 2:CCGTTCCTGTTTACCCTTGA