| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.077494 |
| Chromosome: | chromosome 8 |
| Location: | 1984211 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g367900 | intron|outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGACTCACTGTGCACAGAGTCACACAGACT |
| Internal bar code: | CTGCTCCCCCGTCAGCCGCCCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 436 |
| LEAP-Seq percent confirming: | 94.6855 |
| LEAP-Seq n confirming: | 1942 |
| LEAP-Seq n nonconfirming: | 109 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATATGCTGGCATTTTGCAC |
| Suggested primer 2: | AAAGCCACAAACTCACCACC |