Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.077514 |
Chromosome: | chromosome_12 |
Location: | 8873599 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre12.g544700 | PUF3 | Puf protein | antisense | 5'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TGGGTGCCGCCCTGGGATGGGGGGCGGCGG |
Internal bar code: | ATCGCGCCGTTTTTCATGACGA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 1151 |
LEAP-Seq percent confirming: | 99.6462 |
LEAP-Seq n confirming: | 6759 |
LEAP-Seq n nonconfirming: | 24 |
LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GGCCTTGACGAAACCATTTA |
Suggested primer 2: | AATCCGTGGTAACCTTGCAG |