| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.077529 |
| Chromosome: | chromosome 3 |
| Location: | 1871186 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g154850 | COQ4 | Ubiquinone biosynthesis protein; (1 of 1) K18586 - ubiquinone biosynthesis protein COQ4 (COQ4) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCATGTGCCGCGACCGCGGGGTCGCTGCAT |
| Internal bar code: | CGTTCGTATGGTCATTCTCGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 692 |
| LEAP-Seq percent confirming: | 99.7556 |
| LEAP-Seq n confirming: | 7346 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTACTACGAGCGCCACTTCC |
| Suggested primer 2: | CCGAAGTCCTGAAACAAAGC |