Insertion junction: LMJ.RY0402.077533_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.077533
Chromosome:chromosome 17
Location:2961259
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre17.g720100 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCTGCCACAAGGTGATAGCTGGTGTACTCG
Internal bar code:GCGGTACGCGCCCTGTAACGAA

Confirmation - LEAP-Seq

LEAP-Seq distance:345
LEAP-Seq percent confirming:91.1661
LEAP-Seq n confirming:258
LEAP-Seq n nonconfirming:25
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:GAACGTGTGAACGGTGATTG
Suggested primer 2:GTGTCCACTCCGCATTACCT