| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.077542 |
| Chromosome: | chromosome 9 |
| Location: | 1240577 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g399550 | (1 of 1) PTHR10857//PTHR10857:SF11 - COPINE // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCGCGCATCTGCGCAGTCGCGCCGCCCAC |
| Internal bar code: | ACCTGCCATCCCTTCGACGTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 446 |
| LEAP-Seq percent confirming: | 99.8152 |
| LEAP-Seq n confirming: | 3240 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGAGATGGGGGTTACATCC |
| Suggested primer 2: | CTCGGAGCAGTCTCGTTACC |