| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.077545 |
| Chromosome: | chromosome 3 |
| Location: | 5564015 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g186500 | HAT2 | Histone acetyltransferase 2; (1 of 1) K11303 - histone acetyltransferase 1 (HAT1, KAT1) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTGATTGAACTGCACCGGTGCAGTGACTG |
| Internal bar code: | AGAGCAGTGAGGCGTAGCGTCTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 606 |
| LEAP-Seq percent confirming: | 98.7584 |
| LEAP-Seq n confirming: | 4136 |
| LEAP-Seq n nonconfirming: | 52 |
| LEAP-Seq n unique pos: | 35 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGACGCAGGTGTGGATATG |
| Suggested primer 2: | ATCGTGACAGGTATGGCACA |